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| Benzekri, Hicham; Armesto, Paula; Cousin, Xavier; Rovira, Mireia; Crespo, Diego; Alejandro Merlo, Manuel; Mazurais, David; Bautista, Rocio; Guerrero-fernandez, Dario; Fernandez-pozo, Noe; Ponce, Marian; Infante, Carlos; Zambonino, Jose Luis; Nidelet, Sabine; Gut, Marta; Rebordinos, Laureana; Planas, Josep V.; Begout, Marie-laure; Gonzalo Claros, M.; Manchado, Manuel. |
| Background Senegalese sole (Solea senegalensis) and common sole (S. solea) are two economically and evolutionary important flatfish species both in fisheries and aquaculture. Although some genomic resources and tools were recently described in these species, further sequencing efforts are required to establish a complete transcriptome, and to identify new molecular markers. Moreover, the comparative analysis of transcriptomes will be useful to understand flatfish evolution. Results A comprehensive characterization of the transcriptome for each species was carried out using a large set of Illumina data (more than 1,800 millions reads for each sole species) and 454 reads (more than 5 millions reads only in S. senegalensis), providing coverages ranging from... |
| Tipo: Text |
Palavras-chave: Soles; Transcriptome; Microarray; Orthology; Molecular markers; SoleaDB. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00238/34918/33626.pdf |
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| Carballo, Carlos; Berbel, Concha; Guerrero-cozar, Israel; Jimenez-fernandez, Eduardo; Cousin, Xavier; Begout, Marie-laure; Manchado, Manuel. |
| Internal electronic tagging is a major issue in flatfish species due to the small size of abdominal cavity. In this study, three tag types, referred to as Nonatec, nano and mini, were evaluated in three weight classes of Senegalese sole: small (0.3 g), middle (0.8 g) and large (2.0 g). Tags were injected from the blind side and fish were carefully handled to minimize sharp movements. Tag losses were 8% in the small size class, between 5.2 and 15.1% in the middle size class and 2–4% in the large size class. The mortality rates ranged between 2.0 and 15.0% with the lowest values in the large size class. No negative effects of tags on growth (tagged vs non-tagged fish using a middle size class) were found after 57 days of culture. Four additional trials using... |
| Tipo: Text |
Palavras-chave: Flatfish; PIT-tags; Loss rates; Sex effect; Growth. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00439/55090/56532.pdf |
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| Armesto, Paula; Infante, Carlos; Cousin, Xavier; Ponce, Marian; Manchado, Manuel. |
| In the present work, seven genes encoding Na+,K+-ATPase (NKA) β-subunits in the teleost Solea senegalensis are described for the first time. Sequence analysis of the predicted polypeptides revealed a high degree of conservation with those of other vertebrate species and maintenance of important motifs involved in structure and function. Phylogenetic analysis clustered the seven genes into four main clades: β1 (atp1b1a and atp1b1b), β2 (atp1b2a and atp1b2b), β3 (atp1b3a and atp1b3b) and β4 (atp1b4). In juveniles, all paralogous transcripts were detected in the nine tissues examined albeit with different expression patterns. The most ubiquitous expressed gene was atp1b1a whereas atp1b1b was mainly detected in osmoregulatory organs (gill, kidney and... |
| Tipo: Text |
Palavras-chave: Senegalese sole Na+; K+-ATPase Beta subunit Paralogs Osmoregulation. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00240/35173/35782.pdf |
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| Armesto, Paula; Campinho, Marco A.; Rodríguez-rúa, Ana; Cousin, Xavier; Power, Deborah M.; Manchado, Manuel; Infante, Carlos. |
| In the present work, five genes encoding different Na+,K+ ATPase (NKA) α-isoforms in the teleost Solea senegalensis are described for the first time. Sequence analysis of predicted polypeptides revealed a high degree of conservation across teleosts and mammals. Phylogenetic analysis clustered the five genes into three main clades: α1 (designated atp1a1a and atp1a1b), α2 (designated atp1a2) and α3 (designated atp1a3a and atp1a3b) isoforms. Transcriptional analysis in larvae showed distinct expression profiles during development. In juvenile tissues, the atp1a1a gene was highly expressed in osmoregulatory organs, atp1a2 in skeletal muscle, atp1a1b in brain and heart and atp1a3a and atp1a3b mainly in brain. Quantification of mRNA abundance after a salinity... |
| Tipo: Text |
Palavras-chave: Senegalese sole Na+; K+ ATPase atp1a1a atp1a1b atp1a2 atp1a3a atp1a3b osmoregulation. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00195/30640/29093.pdf |
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